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Image Search Results
Journal: Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Article Title: Activated platelets and platelet-leukocyte aggregates in the equine systemic inflammatory response syndrome
doi: 10.1177/10406387221077969
Figure Lengend Snippet: Cytometric gating protocol. A. Platelet gate (P1) on the basis of the forward (FSC) and side scatter (SSC), threshold FSC 20,000. B. Single-parameter fluorescence histogram of the APC-conjugated polyclonal goat anti-mouse antibody as isotype-negative control for CD41/61 (FL-4: 640 nm, filter 675/25 nm). C. Single-parameter fluorescence histogram gated for APC-conjugated monoclonal mouse anti-sheep antibodies against CD41/61. D. Dotplot representing platelet expression of CD41/61 ( y -axis; FL-4; Q1-UL indicating positive events for CD41/61) and expression of CD11a/18 (leukocytes; x -axis; FL-1, 488 nm, filter 530/30 nm; Q1-LR indicating positive events for CD 11a/18); dual-labeled events positive for CD41/61 and CD11a/18 representing 3.5% platelet-leukocyte aggregates in Q1-UR.
Article Snippet: Matched isotype antibodies were used for negative controls:
Techniques: Fluorescence, Negative Control, Expressing, Labeling